The word "ligate" is spelled with a "g" even though it sounds like "j". This is because "g" can also have a "j" sound when it comes before an "i" or "e" followed by another vowel. In IPA phonetic transcription, this would be represented as /lɪˈɡeɪt/. The verb "ligate" means to tie or bind with a ligature, typically in the context of surgery or medicine. It is important to pay attention to spelling and pronunciation in technical fields to ensure clear communication.
Ligate is a verb that is primarily used in the field of medicine and biochemistry. It refers to the process of joining or connecting two separate strands of DNA or other molecules together using a special enzyme known as a ligase. This process involves the formation of a covalent bond between two adjacent nucleotides, resulting in a tightly connected strand.
In molecular biology, ligation plays a crucial role in various experimental techniques, such as cloning, genetic engineering, and the study of protein-protein interactions. It allows scientists to manipulate and modify genetic material by fusing together specific DNA fragments. By ligating segments of DNA, researchers can create recombinant DNA molecules or construct gene sequences that express desired traits.
The process of ligation involves the use of a ligase enzyme, which catalyzes the formation of a phosphodiester bond between the 3' hydroxyl end of one nucleotide and the 5' phosphate group of another nucleotide. Ligases are highly specific in terms of their substrate preferences, which enables researchers to precisely control the connections between DNA fragments.
Overall, the concept of ligate revolves around the act of joining or linking together molecules, particularly DNA strands, through the use of a ligase enzyme. It is an essential technique in molecular biology and biochemistry that allows for the manipulation and customization of genetic material, ultimately contributing to advancements in various scientific research fields.
To apply a ligature; to constrict a blood-vessel or the pedicle of a tumor by means of a tightly tied thread or fillet.
A practical medical dictionary. By Stedman, Thomas Lathrop. Published 1920.
The word ligate comes from the Latin verb ligare, which means to bind or to tie.