Isoleucyl tRNA Synthetase is a long and complex term, but it can be broken down phonetically to make its spelling easier to understand. The first part, "isoleucyl," is spelled /aɪ-səʊ-ˈliː-səl/. The second part, "tRNA," is spelled /tiː ɑːr en ˈeɪ/. The final part, "synthetase," is spelled /ˈsɪnθəteɪz/. Overall, this term is pronounced as /aɪsəʊˈluːsɪl tiː ɑːr en ˈeɪ sɪnθəteɪz/. It refers to an enzyme found in cells that helps to create a specific type of RNA molecule.
Isoleucyl tRNA synthetase, also known as IleRS, is an enzyme that plays a crucial role in protein synthesis. Specifically, it is responsible for attaching the amino acid isoleucine to its corresponding transfer RNA (tRNA) molecule. This process, known as aminoacylation or charging, ensures that the correct amino acid is incorporated into the growing polypeptide chain during translation.
The enzyme works by recognizing and binding to both the isoleucine amino acid and the specific tRNA molecule that carries the corresponding anticodon sequence. IleRS then catalyzes the transfer of isoleucine from its amino acid pool to the tRNA molecule, forming an aminoacyl-tRNA complex. This charged tRNA molecule is subsequently utilized by the ribosome during translation, where it ensures the accurate incorporation of isoleucine into the protein sequence.
Isoleucyl tRNA synthetase is highly specific for isoleucine, meaning it selectively binds this particular amino acid and attaches it only to the appropriate tRNA species with the matching anticodon sequence. This specificity is essential for maintaining the fidelity of protein synthesis, as mistakes during aminoacylation could result in the incorrect amino acid being incorporated into the growing polypeptide chain, leading to structural and functional defects in the final protein product.
Overall, the function of isoleucyl tRNA synthetase is vital for the accurate and efficient production of proteins in living organisms, ensuring the precise and correct assembly of the amino acid sequence in accordance with the genetic code.