INVERSE PCR Meaning and
Definition
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Inverse PCR (Inverse Polymerase Chain Reaction) is a molecular biology laboratory technique that is used to amplify unknown DNA sequences that flank a known DNA region. It is based on the principle of the conventional Polymerase Chain Reaction (PCR) but works in the opposite direction.
Inverse PCR is primarily employed when only one end of a DNA sequence is known, and the other end needs to be elucidated. It is particularly useful for analyzing sequences that lie adjacent to known genomic regions, such as insertions, deletions, or integrations. This method involves several steps, including digestion of genomic DNA with a restriction enzyme, self-ligation of the digested DNA fragments, and subsequent amplification of the flanking sequence using primers designed from the known region.
Once the unknown DNA regions are amplified through the inverse PCR process, the resulting products can be further analyzed by DNA sequencing, cloning, or other downstream molecular techniques. Inverse PCR is a powerful tool in molecular biology and has applications in various fields, such as genomics, genetics, and functional genomics.
This technique allows for the identification and characterization of unknown DNA sequences within a genome and helps in understanding genetic rearrangements, gene fusions, and other structural alterations. Overall, inverse PCR offers a valuable approach for studying and elucidating DNA sequences that are adjacent to known regions, enabling a deeper exploration of the genome.
Common Misspellings for INVERSE PCR
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Etymology of INVERSE PCR
The word "inverse PCR" is derived from combining two terms: "Inverse" and "PCR".
1. Inverse: The term "inverse" is used here in the sense of reversed or opposite. In the context of PCR (Polymerase Chain Reaction), the term "inverse" refers to a modified or alternative version of the standard PCR technique.
2. PCR: PCR stands for Polymerase Chain Reaction. It is a widely used method in molecular biology to amplify a specific segment of DNA by repeated cycles of heating, cooling, and replication using a DNA polymerase enzyme.
When these two terms are combined, "Inverse PCR" refers to a modified or reversed version of the standard PCR technique, where the target DNA sequence is known but the flanking regions are unknown. It is used for amplifying DNA segments located adjacent to a known DNA sequence.
