How Do You Spell INDIRECT FLUORESCENT ANTIBODY TECHNIQUE?

Pronunciation: [ɪnda͡ɪɹˈɛkt flʊ͡əɹˈɛsənt ˈantɪbˌɒdi tɛknˈiːk] (IPA)

The Indirect Fluorescent Antibody Technique is a common immunological test used to detect the presence of antibodies in a patient sample. The phonetic transcription of the word is /ˌɪndɪˈrɛkt flʊəˈrɛsənt ˈæntɪbɒdi ˈtɛknik/, which helps to explain its spelling. The stress falls on the third syllable of indirect and the first syllable of fluorescent. The word antibody is pronounced with the stress falling on the second syllable, while technique is stressed on the first syllable. Overall, the word is spelled phonetically based on its pronunciation.

Meaning and Definition
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INDIRECT FLUORESCENT ANTIBODY TECHNIQUE Meaning and Definition

  1. The indirect fluorescent antibody technique is a laboratory method used to detect and identify specific antigens or antibodies in biological samples such as blood or tissue samples. It is an immunological test that utilizes fluorescently labeled antibodies to provide a visual indication of the presence and localization of a specific antigen or antibody.

    In this technique, a primary antibody that binds to the target antigen is first applied to the sample. This primary antibody recognizes and attaches itself to the antigen of interest. Then, a secondary antibody that is labeled with a fluorescent dye (such as fluorescein isothiocyanate or rhodamine) is added to the sample. This secondary antibody recognizes and binds to the primary antibody, forming a complex.

    After incubation and washing steps to remove any unbound antibodies, the sample is examined under a fluorescent microscope. When excited by specific wavelengths of light, the fluorescent dye emits bright, visible light, indicating the presence and location of the specific antigen or antibody in the sample.

    The indirect fluorescent antibody technique is particularly valuable in the fields of medical diagnosis, immunology, and microbiology. It allows for the detection of a wide range of specific antigens, such as infectious agents, autoantibodies, and tumor markers, aiding in the diagnosis of various diseases and conditions. It is a sensitive and versatile technique that provides valuable information about the presence, distribution, and concentration of target antigens or antibodies in biological samples.

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