IN SITU HYBRIDISATION Meaning and
Definition
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In situ hybridization is a molecular biology technique used to identify and locate specific nucleic acid sequences within cells or tissues. It involves the use of complementary nucleic acid probes labeled with a detectable marker, such as a fluorescent dye or a radioactive tag, to bind to the target sequences of interest.
The technique allows researchers to study the spatial distribution and expression patterns of specific genes or DNA sequences within intact biological samples. By using complementary probes, in situ hybridization enables the visualization of the target sequences directly in their original location within individual cells or tissues. This provides valuable information about gene expression, cellular localization, and the interaction between genes and cellular structures.
In situ hybridization can be performed on various types of biological samples, ranging from cultured cells and thin sections of tissue to whole-mount embryos. It can be used in both fixed and frozen specimens, and the specificity and sensitivity of the technique can be enhanced through various modifications, such as the use of signal amplification systems.
This technique has emerged as an essential tool in various fields of research, including developmental biology, genetics, oncology, and neuroscience, as it allows scientists to investigate the function and regulation of specific genes within the complex cellular context. Overall, in situ hybridization is a powerful method for visualizing and analyzing genetic information within living organisms.
Common Misspellings for IN SITU HYBRIDISATION
- un situ hybridisation
- jn situ hybridisation
- kn situ hybridisation
- on situ hybridisation
- 9n situ hybridisation
- 8n situ hybridisation
- ib situ hybridisation
- im situ hybridisation
- ij situ hybridisation
- ih situ hybridisation
- in aitu hybridisation
- in zitu hybridisation
- in xitu hybridisation
- in ditu hybridisation
- in eitu hybridisation
- in witu hybridisation
- in sutu hybridisation
- in sjtu hybridisation
- in sktu hybridisation
- in sotu hybridisation
