IMMUNOFLUOROMETRIC ASSAY Meaning and
Definition
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An immunofluorometric assay (IFA) is a laboratory technique used to detect and measure specific substances, typically proteins or biomarkers, in biological samples. It is a highly sensitive and accurate method that combines the principles of immunology and fluorescence detection.
In an IFA, a specific antibody is used to bind and capture the target substance of interest in the sample. This antibody is labeled with a fluorescent molecule, which emits light of a specific wavelength when excited by an external light source, such as a laser beam. The captured target substance is then detected and quantified based on the intensity of the emitted fluorescence.
The IFA procedure involves several steps. First, the biological sample is treated to isolate the target substance and eliminate interference from other components. Then, the sample is incubated with the labeled antibody, allowing the antibody to bind specifically to the target substance. After a washing step to remove unbound molecules, the fluorescence signal is measured using a specialized instrument, such as a fluorometer.
IFA is widely used in clinical diagnostics and research to detect a range of substances, including hormones, tumor markers, infectious agents, and autoimmune antibodies. Its high sensitivity and specificity make it particularly useful for detecting low concentrations of substances in complex biological samples.
Overall, immunofluorometric assay plays a vital role in advancing scientific knowledge, elucidating disease mechanisms, and facilitating accurate diagnosis and monitoring of various conditions.
Common Misspellings for IMMUNOFLUOROMETRIC ASSAY
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Etymology of IMMUNOFLUOROMETRIC ASSAY
The term "immunofluorometric assay" (IFA) is a combination of three key components: "immuno-", "fluoro-", and "metric assay".
1. "Immuno-" is derived from the word "immunity", which is derived from the Latin word "immunitas", meaning exemption or privilege. This prefix is often used in scientific and medical terms related to the immune system or immunity.
2. "Fluoro-" is derived from the word "fluorescence", which is derived from the Latin word "fluere", meaning to flow. Fluorescence refers to the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is often used in scientific terms related to the utilization of fluorescent dyes or markers.
3. "Metric assay" refers to a method or technique for measuring or quantifying something.
