How Do You Spell HORSERADISH PEROXIDASE III?

Pronunciation: [hˈɔːsɹɐdˌɪʃ pˈɛɹəksˌɪde͡ɪs ɹˌə͡ʊmən θɹˈiː] (IPA)

Horseradish peroxidase III is an enzyme commonly used in biomedical research. The correct spelling of the word is pronounced as /ˈhɔrsrædɪʃ pəˈrɒksɪˌdeɪs ˌθriː/. However, some people may find it difficult to spell or pronounce due to the unusual combination of letters and sounds. The IPA phonetic transcription of the pronunciation shows that the emphasis is on the first syllable with a slight emphasis on the last syllable. Despite the challenging spelling, horseradish peroxidase III plays an essential role in many scientific experiments.

HORSERADISH PEROXIDASE III Meaning and Definition

  1. Horseradish peroxidase III (HRP III) is an enzyme derived from the horseradish plant (Armoracia rusticana) that belongs to the peroxidase family. It is specifically classified as a member of the Class III peroxidase subgroup. This enzyme is widely used in biochemistry and molecular biology research due to its exceptional catalytic properties and stability.

    HRP III is an iron-containing heme protein that catalyzes the oxidation of a wide range of substrates, such as phenols, aromatic amines, and thiols, using hydrogen peroxide as an electron acceptor. In the presence of its substrate and hydrogen peroxide, HRP III undergoes a reaction cycle that results in the formation of two highly reactive intermediates, compound I and compound II, which contribute to the enzyme's strong oxidizing activity.

    The enzyme is a tetramer composed of four identical subunits, each containing a heme moiety responsible for its peroxidase activity. HRP III has a molecular weight of approximately 38,000 Da and a relatively neutral isoelectric point of pH 6.4.

    Due to its versatility, HRP III is commonly utilized as a label or marker enzyme in various applications, including immunoassays, enzyme-linked immunosorbent assays (ELISA), and Western blotting. Its ability to produce colored or luminescent reaction products makes it a valuable tool for detection and visualization purposes. Additionally, HRP III is commonly conjugated to antibodies or other molecules of interest to enable specific targeting and detection in biological and biochemical assays.

Common Misspellings for HORSERADISH PEROXIDASE III

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