HEPES Meaning and
Definition
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HEPES (2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid) is a white crystalline solid that is frequently used as a buffering agent in biochemical and biological research. It is categorized as a zwitterionic amine-based buffer due to its ability to exist in both a cationic and anionic form.
HEPES is commonly utilized in cell culture media, diagnostic assays, and protein biochemistry applications due to its capacity to maintain a stable pH environment. This buffering system is advantageous because it is highly soluble in water and exhibits a pH range of approximately 6.8 to 8.2, making it suitable for use in physiological conditions.
In addition to its buffering capabilities, HEPES is known for being chemically and enzymatically inert, which ensures that it does not interfere with the biological processes or assays being performed. These properties make HEPES a reliable choice for maintaining cell viability and enhancing the accuracy and reproducibility of experiments.
HEPES is generally prepared by dissolving the appropriate amount in deionized water to achieve the desired concentration. It is often used in combination with other buffering agents to create customized buffer systems, depending on the specific experimental requirements.
Overall, HEPES is an essential chemical compound for maintaining optimal pH conditions in various biological applications, offering researchers a reliable method to control experimental variables and ensure accurate and reproducible results.
Common Misspellings for HEPES
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