GRAMS SOLUTION Meaning and
Definition
-
Gram's solution is a commonly used chemical solution extensively utilized in microbiology for the Gram staining technique. It is considered a differential staining method that enables the differentiation of various types of bacteria based on their cell wall properties. The solution is named after the Danish bacteriologist Hans Christian Gram, who developed this staining technique in the late 19th century.
Gram's solution comprises various components in specific concentrations, including crystal violet dye, iodine, ethanol or acetone, and safranin counterstain. The crystal violet dye serves as the primary stain that imparts a purple color to both Gram-negative and Gram-positive bacteria. The iodine acts as a mordant, which strengthens the staining properties of the crystal violet by forming a crystal violet-iodine complex. The next step involves the use of ethanol or acetone as a decolorizing agent. It washes away the crystal violet-iodine complex from the Gram-negative bacteria, making them appear colorless. Gram-positive bacteria, on the other hand, retain the purple dye due to the presence of a thicker peptidoglycan layer in their cell walls. Finally, safranin counterstain is applied to the decolorized Gram-negative bacteria, giving them a pink color.
In summary, Gram's solution is a chemical solution used in the Gram staining technique to distinguish between different types of bacteria based on the staining properties of their cell walls. It plays a crucial role in microbiology and aids in the identification and classification of bacteria.
Common Misspellings for GRAMS SOLUTION
- frams solution
- vrams solution
- brams solution
- hrams solution
- yrams solution
- trams solution
- geams solution
- gdams solution
- gfams solution
- gtams solution
- g5ams solution
- g4ams solution
- grzms solution
- grsms solution
- grwms solution
- grqms solution
- grans solution
- graks solution
- grajs solution
- grama solution
Etymology of GRAMS SOLUTION
The term "Gram's solution" is named after Hans Christian Gram, a Danish bacteriologist. He developed a staining technique called the Gram stain, which is widely used in microbiology to differentiate bacteria into two groups based on their cell wall structure. Gram-positive bacteria retain the purple crystal violet stain, while Gram-negative bacteria do not and appear red when counterstained with a contrasting dye. "Gram's solution" refers to the specific combination of stains and solvents used in the Gram stain technique, as initially described by Gram in 1884.
