GRAM METHOD Meaning and
Definition
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The term "gram method" refers to a systematic approach used in microbiology and laboratory medicine for the identification and classification of bacteria based on their characteristics, particularly the Gram stain. The Gram method, also known as the Gram staining technique, is named after the Danish bacteriologist Hans Christian Gram who developed it in the late 19th century.
The Gram method involves a series of staining steps to differentiate bacteria into two major groups: Gram-positive and Gram-negative. During the process, a sample of bacteria is first fixed to a glass slide and then stained with crystal violet dye followed by iodine solution. The slide is then decolorized with a mix of ethanol and acetone, washed, and stained with a counterstain like safranin. The result is the classification of bacteria into either Gram-positive (retaining the violet stain) or Gram-negative (turning pink or red with the counterstain).
The Gram method is a fundamental tool in clinical microbiology and helps in the initial identification of bacteria, as well as guiding treatment decisions by determining antibiotic susceptibility. Many important bacterial species can be distinguished based on the Gram reaction, as it reflects structural differences in the cell wall composition. In addition, the Gram method plays a crucial role in the diagnosis of infections and the understanding of the microbiota in various fields of research, such as environmental microbiology and food safety.
Common Misspellings for GRAM METHOD
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Etymology of GRAM METHOD
The word "gram method" does not have its own etymology, as it is a combination of two separate terms: "gram" and "method".
- "Gram" is derived from the Greek word "gramma", meaning "a letter" or "something written". It is also related to the Latin word "gramma", which has a similar meaning. In English, "gram" generally refers to a unit of weight, which is based on the metric system and is equal to one thousandth of a kilogram.
- "Method" comes from the Latin word "methodus", which is borrowed from the Greek word "methodos", meaning "pursuit" or "following after". It refers to a systematic way or procedure of doing something, often used to achieve a specific goal or result.
Similar spelling words for GRAM METHOD
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