GINI ENDONUCLEASE Meaning and
Definition
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GinI endonuclease is a type II restriction enzyme commonly found in the bacterium Geobacillus stearothermophilus. It belongs to the endonuclease superfamily, which are enzymes that cleave DNA at specific recognition sites. GinI endonuclease recognizes and cuts DNA at the sequence site 5'-GGATG-3'.
As a type II restriction enzyme, GinI endonuclease functions as a homodimer composed of two identical subunits. Each subunit in the dimer possesses an independent DNA-binding domain, which allows the enzyme to bind to its recognition site on the DNA sequence. Once bound, the enzyme catalyzes the hydrolysis of the phosphodiester bond on both DNA strands, resulting in double-strand breaks.
The cleavage catalyzed by GinI endonuclease generates two DNA fragments with cohesive ends, i.e., the ends are complementary to each other and can easily re-join using DNA ligase. The cohesive ends can also anneal with other compatible sequences, allowing for DNA manipulation techniques such as DNA cloning and genetic engineering.
GinI endonuclease has a preference for a specific temperature range, thriving in thermophilic conditions. This attribute makes it useful in laboratory procedures that require stable activity at high temperatures.
Overall, GinI endonuclease plays a vital role in DNA manipulation techniques and gene editing technology due to its ability to specifically cut DNA at defined recognition sites.
Common Misspellings for GINI ENDONUCLEASE
- fini endonuclease
- vini endonuclease
- bini endonuclease
- hini endonuclease
- yini endonuclease
- tini endonuclease
- guni endonuclease
- gjni endonuclease
- gkni endonuclease
- goni endonuclease
- g9ni endonuclease
- g8ni endonuclease
- gibi endonuclease
- gimi endonuclease
- giji endonuclease
- gihi endonuclease
- ginu endonuclease
- ginj endonuclease
- gink endonuclease
- gino endonuclease
Etymology of GINI ENDONUCLEASE
The word "GinI" comes from the name of the bacterium in which the endonuclease was first discovered. The bacterium is called "Geobacillus stearothermophilus" (often abbreviated as "G. stearothermophilus"). The abbreviation "GinI" is derived from the first letters of the genus "G" and species "i" of the bacterium's scientific name.
The term "endonuclease" is a combination of two words: "endo" meaning "within" or "inside" and "nuclease" which refers to an enzyme that breaks down nucleic acids (DNA or RNA). Therefore, an endonuclease refers to an enzyme that cuts nucleic acids at specific internal points.
