The Gelle test, also known as the gel precipitation test, is a diagnostic tool used to determine the presence of antibodies in biological samples such as blood or saliva. The word "Gelle" is pronounced with a soft "g" sound, represented in IPA phonetic transcription as "dʒɛl". The second syllable is pronounced like the word "bell" and the stress is on the first syllable. It is important to spell the word correctly in medical contexts to ensure clear communication and accurate diagnosis.
The Gelle test, also known as the Giemsa-ELISA or Giemsa enzyme-linked immunosorbent assay, is a laboratory diagnostic test utilized for the detection of antibodies against certain pathogens, particularly tick-borne diseases. This test is primarily performed to diagnose diseases caused by spirochetes, such as Lyme disease, relapsing fever, and certain other tick-borne infections.
During the Gelle test, patient serum or blood sample is collected and mixed with antigens derived from the pathogen being tested for. These antigens are then immobilized onto a solid substrate, such as a microplate, and any specific antibodies present in the patient's sample will bind to the corresponding antigens. After several washing steps to remove any unbound components, an enzyme-labeled secondary antibody is added, which will attach to the patient's antibodies if present. Eventually, a colorimetric substrate is added and the resulting color change is measured.
The Gelle test is based on the principle of enzyme-linked immunosorbent assay (ELISA) and utilizes the Giemsa staining technique, which helps in visualizing the reaction results. This test is highly sensitive and specific, aiding in the accurate diagnosis of tick-borne diseases. It allows healthcare professionals to determine if a person has been exposed to a specific pathogen and has developed an immune response against it. The Gelle test is widely used in clinical and research settings, contributing to the prompt identification and management of tick-borne infections.
A vibrating tuning-fork is applied over the mastoid process; if it is heard, the air in the external auditory canal is compressed, thereby fixing the stapes in the oval window, and the sound ceases to be heard, but is again perceived if the air pressure is removed; a test of the mobility of the ossicles.
A practical medical dictionary. By Stedman, Thomas Lathrop. Published 1920.