FOLINS TEST Meaning and
Definition
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Folin's test refers to a chemical analysis technique used to determine the concentration of reducing substances such as sugars, aldehydes, and aromatic compounds in a given sample. It was developed by Otto Folin, an American biochemist, and has found significant applications in various fields, particularly in clinical and food analysis.
The Folins test involves the addition of Folin-Ciocalteu reagent to the sample solution, followed by an alkaline solution such as sodium carbonate. The Folin-Ciocalteu reagent consists of phosphomolybdic and phosphotungstic acids, which undergo a redox reaction with the reducing substances present in the sample, resulting in the formation of a blue-colored complex. The intensity of the blue color is directly proportional to the concentration of the reducing substances in the sample.
This test is commonly used in the determination of total phenolic content in various plant-based products, including fruits, vegetables, and beverages. It is also used in the analysis of carbohydrates, such as glucose and fructose, in food and clinical samples. Furthermore, the Folins test is extensively utilized in research and quality control laboratories to assess the antioxidant capacity of natural compounds and evaluate the stability and oxidation state of organic substances.
Overall, the Folins test is a reliable and widely employed analytical technique that provides valuable information regarding the reducing substances in a given sample, thus aiding in the characterization and quality assessment of various products.
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Etymology of FOLINS TEST
The word "Folin's test" is derived from the name of the American biochemist Otto Folin. Otto Folin (1867-1934) was a renowned scientist known for his significant contributions to the field of analytical chemistry and medical research.
Folin developed various chemical tests and methodologies for determining the composition and concentration of substances in biological samples. The Folin's test refers to a specific chemical test called the Folin-Ciocalteu reagent method, which is used to estimate the total phenolic content in a given sample.
This test involves the addition of Folin-Ciocalteu reagent to the sample, which reacts with phenolic compounds, forming a blue-colored complex. The intensity of the color is proportional to the concentration of phenolic compounds present in the sample.
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