FLUOROPHOTOMETER Meaning and
Definition
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A fluorophotometer is a specialized scientific instrument used to measure the fluorescent properties of a specific sample solution. It is mainly employed in fields like life sciences, biochemistry, molecular biology, and clinical research.
The instrument typically consists of a source of light, such as a xenon lamp, which emits a broad spectrum of wavelengths. The light is directed towards the sample, and when it interacts with the molecules within the sample, it can excite certain fluorophores (fluorescent molecules present in the sample). These excited fluorophores then emit fluorescence of a distinct wavelength.
The fluorophotometer measures the intensity of this emitted fluorescence and records it as a function of wavelength. It analyzes the spectral characteristics, including the emission wavelength and intensity, to determine various properties of the sample, such as the concentration or specific activity of the fluorophores.
Fluorophotometers offer high sensitivity and accuracy, allowing scientists to conduct quantitative analysis of fluorescent samples. They are equipped with filters or monochromators that selectively transmit specific wavelengths of light, enabling the precise measurement of fluorescence emission at the desired wavelengths. Additionally, they often have software programs that control instrument settings, collect data, and perform calculations for analysis.
Overall, fluorophotometers have become indispensable tools in many scientific disciplines, playing a crucial role in studying biomolecules, analyzing molecular interactions, and advancing our understanding of various biological processes.
Common Misspellings for FLUOROPHOTOMETER
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Etymology of FLUOROPHOTOMETER
The term "fluorophotometer" is derived from two main components: "fluoro-" and "photometer".
The prefix "fluoro-" comes from the Greek word "fluoros", meaning "flowing" or "flux". In modern usage, it usually refers to the fluorescence phenomenon, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation.
The word "photometer" is derived from two Greek words: "phos", meaning "light", and "metron", meaning "measure". A photometer is an instrument used to measure the intensity of light or other electromagnetic radiation.
Combining these two elements, the word "fluorophotometer" refers to an instrument specifically designed to measure and analyze fluorescence. It is utilized to quantify the intensity and characteristics of the light emitted by fluorescent substances.
