The spelling of "fluorimeter" can be explained through its IPA phonetic transcription, [flʊərɪˈmɛtər]. The first syllable "flu" is pronounced as "flʊ," with a short "u" sound. The second syllable "ori" is pronounced as "ərɪ," with a schwa sound followed by a short "i" sound. The final syllable "meter" is pronounced as "ˈmɛtər," with the stress on the first syllable and a short "e" sound followed by an "ɚ" sound. The word refers to a device used to measure the fluorescence of a substance.
A fluorimeter is a scientific instrument used to measure and analyze the fluorescent properties of substances. It is a highly sensitive device that helps identify and quantify the presence of fluorophores or fluorescent molecules in a sample and assists in various fields such as chemistry, biochemistry, physics, and environmental science.
The main functioning principle of a fluorimeter involves the emission of light of a specific wavelength onto the sample being studied. When the sample absorbs this excitation light, it promotes electrons to a higher energy state. As the electrons return to their original states, they emit fluorescence of a longer wavelength, unique to the specific substance being measured. The instrument then detects and measures this emitted fluorescent light and provides information about the sample's nature and concentration.
Fluorimeters are equipped with filters to selectively isolate the emitted fluorescence from interfering background signals, enhancing the accuracy and precision of measurements. They often utilize photomultiplier tubes or photodiodes as detectors to convert the light signals into electrical current, which can be further processed and analyzed.
The versatility of fluorimeters enables a wide range of applications, including monitoring and quantifying DNA, proteins, enzymes, or ions in biological and chemical samples. They can also determine the concentration of pollutants, trace elements, or drugs in environmental samples or pharmaceutical analysis. Overall, fluorimeters are essential tools for researchers, scientists, and professionals working in laboratories, allowing them to gain valuable insights into the composition and behavior of fluorescent substances.
The word "fluorimeter" comes from the combination of two terms: "fluor-" and "-meter".
1. "Fluor-" is derived from the Latin word "fluor" meaning "flow". It also relates to "fluere", which means "to flow". In this context, "fluor-" refers to fluorescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation.
2. "-Meter" originates from the Greek word "metron" meaning "measure", which has been adopted into English to indicate an instrument used for measuring a specific quantity or property.
Combining these two components, "fluorimeter" refers to an instrument or device used for measuring or detecting fluorescence.