How Do You Spell FLUORESCENCE ACTIVATED CELL SORTINGS?

Pronunciation: [flʊ͡əɹˈɛsəns ˈaktɪvˌe͡ɪtɪd sˈɛl sˈɔːtɪŋz] (IPA)

Fluorescence Activated Cell Sortings, or FACS for short, is a powerful technique used in cellular analysis and sorting. The spelling of "fluorescence" starts with the "fl" blend, followed by the "oo" sound, and then the "r" sound. The "e" at the end is silent. "Activated" is pronounced with a long "a" sound, followed by the "k" and "t" sounds. "Cell" is pronounced with a hard "c" sound and a short "e" sound. "Sorting" starts with the "s" sound and ends with the "ing" sound. The IPA phonetic transcription for "Fluorescence Activated Cell Sortings" is /flʊəˈrɛsns ˈæktɪveɪtɪd sɛl ˈsɔrdɪ

FLUORESCENCE ACTIVATED CELL SORTINGS Meaning and Definition

1. Fluorescence Activated Cell Sorting (FACS) refers to a sophisticated technique used in cell biology and flow cytometry to isolate and separate cells based on their physical and fluorescent characteristics. FACS combines the principles of flow cytometry with the ability to separate subpopulations of cells in a heterogeneous mixture.

   FACS involves tagging cellular components, such as proteins or DNA, with specific fluorescent labels called fluorophores. Cells are then suspended in a fluidic system and passed through a stream of fluid where they pass a laser beam, leading to excitation of the fluorophores. The resulting fluorescence emission is detected and quantified for each individual cell.

   This information can be used to analyze various cellular properties, such as cell surface markers, intracellular proteins, or DNA content. Additionally, FACS can be used to separate cells into distinct populations based on their fluorescent characteristics. This is achieved by incorporating an electrostatic charge and an array of charged plates that defocus cells into separate collection tubes, allowing the isolation of different cell types or subclasses.

   FACS has numerous applications in immunology, cancer research, stem cell research, and microbiology. It enables researchers to investigate and characterize cell populations, identify rare cell subsets, and isolate specific cell types for further analysis or experimental manipulations. This technique has revolutionized the study of cellular heterogeneity, and its high-throughput capabilities have broadened our understanding of diverse biological processes and disease mechanisms.

Common Misspellings for FLUORESCENCE ACTIVATED CELL SORTINGS

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