ENDONUCLEASE LLACI Meaning and
Definition
-
Endonuclease LlaCI is a specific type of restriction endonuclease enzyme that is derived from the bacteria Lactococcus lactis subsp. cremoris. It is commonly used in molecular biology and genetic engineering techniques for precise DNA manipulation.
Endonucleases are enzymes that cleave DNA at specific recognition sites within a DNA molecule. LlaCI is classified as a Type II restriction endonuclease because it recognizes a specific DNA sequence and cleaves it internally at a defined position. The recognition sequence for LlaCI is typically 5'-GC↓GNAGC-3', where the ↓ indicates the cleavage site.
The activity of LlaCI is dependent on certain environmental factors, such as the presence of divalent metal ions like magnesium or calcium. It requires these ions to coordinate its catalytic activity and facilitate the DNA cleavage process.
Endonuclease LlaCI has found extensive use in molecular biology research due to its ability to generate specific DNA fragments with defined ends. These fragments can then be used in techniques such as DNA cloning, genome mapping, and DNA sequencing. LlaCI's cleavage activity is highly specific, as it only recognizes and cleaves DNA that contains its target sequence, which allows for accurate DNA manipulation with minimal non-specific cutting.
Overall, Endonuclease LlaCI serves as a valuable tool in DNA analysis and manipulation, providing researchers with a means to precisely cut DNA molecules at specific sites for various downstream applications.
Common Misspellings for ENDONUCLEASE LLACI
- wndonuclease llaci
- sndonuclease llaci
- dndonuclease llaci
- rndonuclease llaci
- 4ndonuclease llaci
- 3ndonuclease llaci
- ebdonuclease llaci
- emdonuclease llaci
- ejdonuclease llaci
- ehdonuclease llaci
- ensonuclease llaci
- enxonuclease llaci
- enconuclease llaci
- enfonuclease llaci
- enronuclease llaci
- eneonuclease llaci
- endinuclease llaci
- endknuclease llaci
- endlnuclease llaci
- endpnuclease llaci
Etymology of ENDONUCLEASE LLACI
The word "endonuclease" is derived from the Greek roots "endo-" meaning "within" or "inside" and "nuclease" which refers to an enzyme that breaks down nucleic acids (DNA or RNA). "Endonuclease" thus describes an enzyme that cleaves within a nucleic acid molecule.
"LlaCI" is a specific type of endonuclease named after the strain of bacteria from which it was isolated. The "Lla" portion of the name refers to the bacterial species, and "CI" likely denotes its classification as a restriction enzyme. Restriction enzymes, like LlaCI, are endonucleases that recognize specific DNA sequences and cut the DNA at those sites.
