ELLMANS REAGENT Meaning and
Definition
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Ellman's reagent refers to a yellow-colored compound known as 5,5'-dithiobis-(2-nitrobenzoic acid), abbreviated as DTNB. It is commonly employed in chemical analysis and biochemistry to determine the concentration of sulfhydryl groups (also known as thiols) in biological samples. Ellman's reagent is named after its developer, Howard B. Ellman, who introduced it in 1959 as a practical means to assess thiols in various research applications.
Thiols are organic compounds characterized by the presence of a sulfhydryl group (-SH), which plays a crucial role in biological systems. Ellman's reagent reacts with these sulfhydryl groups, resulting in a reaction referred to as a thiol-disulfide exchange reaction. This reaction leads to the formation of a mixed disulfide bond, producing a yellow-colored compound. The intensity of the yellow color can then be measured spectrophotometrically, allowing for the quantification of thiols present in the sample.
Ellman's reagent is highly specific towards thiols, making it a valuable tool in the study of enzyme activity, protein structure, and other biochemical processes. Its sensitivity, simplicity, and reliability have contributed to its widespread use in both academic and industrial research settings. However, it is important to note that Ellman's reagent is light-sensitive and should be stored in a dark, cool place to maintain its stability.
Common Misspellings for ELLMANS REAGENT
- wllmans reagent
- sllmans reagent
- dllmans reagent
- rllmans reagent
- 4llmans reagent
- 3llmans reagent
- eklmans reagent
- eplmans reagent
- eolmans reagent
- elkmans reagent
- elpmans reagent
- elomans reagent
- ellnans reagent
- ellkans reagent
- elljans reagent
- ellmzns reagent
- ellmsns reagent
- ellmwns reagent
- ellmqns reagent
- ellmabs reagent
Etymology of ELLMANS REAGENT
The term "Ellman's reagent" is named after its creator, a British biochemist named Nathan O. Kaplan Ellman. Nathan Ellman developed this reagent in 1958 as a colorimetric agent for measuring thiol (sulfhydryl) groups in biological samples. Since its development, Ellman's reagent has been extensively used for measuring thiol concentrations in various biochemical and biomedical studies.
