DOUBLE DIGESTION Meaning and
Definition
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Double digestion is a molecular biology technique that involves the sequential digestion of DNA with two different restriction enzymes. A restriction enzyme, also known as a restriction endonuclease, is an enzyme that recognizes specific DNA sequences and cuts the DNA at those sites. Double digestion is performed to obtain a specific DNA fragment by cutting the DNA at two different sites simultaneously.
The process of double digestion begins with the selection of two restriction enzymes with different recognition sites that are present in the target DNA. The DNA is first incubated with the first enzyme, which cuts the DNA at its respective recognition site. After the first digestion, a second enzyme is added, which cuts the DNA at its own recognition site. These two cuts generate DNA fragments with defined ends.
Double digestion is useful in various molecular biology applications, such as cloning, DNA fingerprinting, and gene mapping. It allows for the cloning of DNA fragments by generating compatible ends that can be combined with other DNA molecules. Double digestion also aids in DNA fingerprinting by producing different fragment patterns for different individuals, enabling their identification. Furthermore, in gene mapping, double digestion helps determine the relative positions of different genetic markers on a chromosome.
Overall, double digestion serves as a valuable tool in molecular biology, enabling precise manipulation of DNA molecules to achieve specific research objectives.
Common Misspellings for DOUBLE DIGESTION
- souble digestion
- xouble digestion
- couble digestion
- fouble digestion
- rouble digestion
- eouble digestion
- diuble digestion
- dkuble digestion
- dluble digestion
- dpuble digestion
- d0uble digestion
- d9uble digestion
- doyble digestion
- dohble digestion
- dojble digestion
- doible digestion
- do8ble digestion
- do7ble digestion
- douvle digestion
- dounle digestion
Etymology of DOUBLE DIGESTION
The word "double digestion" is primarily used in the field of molecular biology to describe a technique used to cleave DNA molecules using two different restriction enzymes. The etymology of "double digestion" can be understood by breaking down the two constituent words:
1. Double: The word "double" originates from the Old French word "dubler", meaning "to double". It is derived from the Latin word "duplus", meaning "twofold". In this context, "double" refers to the use of two restriction enzymes during the DNA digestion process.
2. Digestion: The term "digestion" comes from the Latin word "digestio", which means "breaking down". It is derived from the Latin verb "digestĕre", meaning "to divide, distribute, separate".
