DHAR stands for "Deoxyribonucleic Acid Hybridization Release assay." It is a sophisticated laboratory technique used to identify and quantify specific DNA sequences in biological samples.
In DHAR, a target DNA sequence is tagged with a labeling molecule, such as a radioactive or fluorescent tag. Then, this labeled DNA is mixed with a sample containing a mixture of DNA molecules. The labeled DNA hybridizes with its complementary sequences, forming a stable DNA duplex.
Next, a releasing agent is added, which specifically disrupts the hybridization between the labeled DNA and its complementary DNA sequences. As a result, only the labeled DNA molecules that have not hybridized with their complementary sequences are released into the surrounding solution.
The released labeled DNA molecules are then separated from the rest of the sample, often through precipitation or filtration techniques. Finally, the quantity of labeled DNA is measured using specialized lab equipment, such as scintillation counters for radioactive tags or fluorimeters for fluorescent tags. The measured quantity reflects the amount of the targeted DNA sequence present in the original sample.
DHAR is widely used in the fields of molecular biology, genetics, and biotechnology. It allows researchers to identify and quantify specific DNA sequences within a complex mixture, aiding various applications such as disease diagnosis, genetic testing, forensic analysis, and environmental monitoring. This technique has significantly contributed to our understanding of DNA and its role in various biological processes.
