Cytohistological preparation technique is a process used in the field of pathology and laboratory medicine to prepare and examine both cellular and tissue specimens for diagnostic purposes. It involves a series of steps aimed at preserving the structure and cellular integrity of the specimen to enable detailed microscopic examination and analysis.
The process starts with the collection of the biopsy or tissue sample, which can be obtained through various methods such as fine needle aspiration, biopsy, or surgical excision. Once the sample is collected, it is processed and prepared for examination under a microscope.
Cytohistological preparation technique typically involves fixation, where the specimen is treated with a fixative solution to preserve the cellular structure. This is followed by processing the specimen through various steps, including dehydration, clearing, and embedding in a suitable medium for support. The specimen is then sectioned thinly using a microtome or cryostat to obtain adequate slices for examination.
After sectioning, the specimen is mounted on a glass slide and may undergo staining with various dyes or histochemical techniques to highlight specific cellular components or structures. Ultimately, the prepared slide is examined under a microscope by a pathologist or trained laboratory personnel to identify and evaluate cellular or tissue abnormalities.
Overall, the cytohistological preparation technique is an integral part of diagnostic pathology as it enables the detailed examination of cellular and tissue specimens, aiding in the identification and characterization of diseases or abnormalities.
