CRNA PROBES Meaning and
Definition
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cRNA probes, also known as complementary RNA probes, are synthetic RNA molecules that are used in molecular biology and genetics research to detect the presence and location of specific RNA sequences in cells and tissues.
These probes are generated in the laboratory by reverse transcribing a DNA template into complementary RNA using the enzyme reverse transcriptase. The DNA template can be obtained from various sources, such as genes of interest or cloned DNA sequences.
cRNA probes are designed to be complementary to the RNA sequence of interest, allowing them to specifically bind to the target RNA and form stable RNA-DNA hybrids via Watson-Crick base pairing. These hybrids can be detected and visualized using different methods, including fluorescence or radioactive labeling, providing researchers with valuable information about the expression level and localization of specific RNA molecules in biological samples.
cRNA probes have diverse applications in molecular biology, including gene expression analysis, in situ hybridization, and northern blotting. By hybridizing to RNA molecules in cells or tissues, cRNA probes can help researchers study gene function, determine the spatial and temporal expression patterns of genes, and investigate the roles of specific RNA molecules in cellular processes or disease states.
In summary, cRNA probes are synthetic RNA molecules that are used in molecular biology research to detect and study the presence, localization, and expression of specific RNA sequences in biological samples.
Common Misspellings for CRNA PROBES
- xrna probes
- vrna probes
- frna probes
- drna probes
- c5na probes
- c4na probes
- crba probes
- crma probes
- crja probes
- crha probes
- crnz probes
- crns probes
- crnw probes
- crnq probes
- crna orobes
- crna lrobes
- crna -robes
- crna 0robes
- crna peobes
- crna pdobes
Etymology of CRNA PROBES
The word "cRNA probes" is an acronym derived from several terms:
1. cRNA: It stands for "complementary RNA". Complementary RNA is synthesized from a complementary DNA (cDNA) template using the enzyme reverse transcriptase. It is often used as a tool in molecular biology to study gene expression, as it can hybridize specifically to the corresponding mRNA (messenger RNA) sequence.
2. Probes: In the context of molecular biology, probes are short, single-stranded DNA or RNA molecules that are designed to bind specifically to a target nucleic acid sequence. They are labeled or tagged with a fluorescent or radioactive marker, allowing their detection.
Therefore, the word "cRNA probes" essentially combines these terms to describe RNA molecules synthesized in a complementary manner to the target mRNA sequence, and used to specifically label and detect that sequence in molecular biology experiments.
