CHROMATOGRAMS Meaning and
Definition
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Chromatograms refer to the visual representations of the separation of components in a mixture obtained through a chromatographic technique. Chromatography is a widely used analytical method that aids in the separation, identification, and quantification of different substances present in a sample. The analysis is based on the differential retention of components in the sample as they interact with the stationary and mobile phases in the chromatographic system.
Chromatograms are typically displayed as graphical plots with the retention time or volume on the x-axis and the response or concentration on the y-axis. The peaks or individual bands observed in the chromatogram correspond to the different components present in the mixture. These peaks provide information about the components' relative concentrations, retention times, and peak shapes, which can be further utilized for qualitative and quantitative analysis.
Depending on the chromatographic technique employed, various types of chromatograms can be generated, such as gas chromatograms (GC), liquid chromatograms (LC), high-performance liquid chromatograms (HPLC), thin-layer chromatograms (TLC), or paper chromatograms.
Interpretation and analysis of chromatograms play a vital role in several scientific and industrial sectors, including pharmaceuticals, environmental monitoring, forensics, food analysis, and biochemical research. By comparing chromatograms of known substances with those of unknowns, scientists can identify and quantify the components present in a sample, aiding in the investigation, quality control, and understanding of complex mixtures.
Etymology of CHROMATOGRAMS
The word chromatograms is derived from the combination of two Greek words: chroma meaning color and graphein meaning to write or to record.
