BIURET TEST Meaning and
Definition
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The biuret test is a chemical test commonly used to determine the presence of proteins in a given solution. It is based on the principle that when proteins react with a copper(II) sulfate solution in an alkaline medium, a violet-colored complex known as biuret is formed. This forms the basis of distinguishing proteins from other compounds.
The test derives its name from the compound biuret, which shares a similar structure to a protein. The biuret complex forms when the peptide bonds in proteins react with copper ions under alkaline conditions, resulting in a color change from blue to violet or purple. This color change is considered a positive result for the presence of proteins. In contrast, if no color change occurs, it indicates the absence of proteins in the tested solution.
The biuret test is a simple and widely used method in laboratories because it provides a quick and reliable indication of protein presence. It is often utilized in biochemistry, molecular biology, and food science. Additionally, the intensity of the color change in the biuret test can be directly proportional to the concentration of proteins present, allowing for quantitative analysis.
However, it is important to note that the biuret test will not differentiate between different types of proteins, as all proteins contain peptide bonds and will therefore react with the test. To distinguish between specific protein types, further tests or techniques are required.
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For urea; caustic soda is added and then a copper sulphate solution drop by drop; the presence of urea is indicated by a pink color, changing to bluish, of the solution.
A practical medical dictionary. By Stedman, Thomas Lathrop. Published 1920.
Common Misspellings for BIURET TEST
- viuret test
- niuret test
- hiuret test
- giuret test
- buuret test
- bjuret test
- bkuret test
- bouret test
- b9uret test
- b8uret test
- biyret test
- bihret test
- bijret test
- biiret test
- bi8ret test
- bi7ret test
- biueet test
- biudet test
- biufet test
- biutet test
Etymology of BIURET TEST
The word "biuret" in the term "biuret test" is derived from the chemical compound called biuret, which is a white crystalline solid. Biuret is named after Friedrich Wöhler, a German chemist, who discovered it in 1828. The compound itself was named using a combination of the Latin word "bi" (meaning two) and "urea" (referring to a compound that was known to contain biuret at the time), indicating its relationship to urea. The biuret test, developed based on the reactivity of biuret with copper ions, is a chemical test used to detect the presence of proteins in a solution.
Similar spelling words for BIURET TEST
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