How Do You Spell AFFINITY CHROMATOGRAPHY?

Pronunciation: [ɐfˈɪnɪti kɹˌə͡ʊmɐtˈɒɡɹəfi] (IPA)

Affinity chromatography is a laboratory technique used to purify proteins or other molecules based on their specific interactions with ligands. The spelling of this word can be explained by using the International Phonetic Alphabet (IPA) transcription. The first syllable "a-f-i" is pronounced as /əˈfɪ/, the second syllable "n-i-t-y" is pronounced as /nɪti/, and the final syllable "c-h-r-o-m-a-t-o-g-r-a-p-h-y" is pronounced as /kroʊməˈtɑɡrəfi/. Overall, the word is pronounced as /əˈfɪnɪti kroʊməˈtɑɡrəfi/.

AFFINITY CHROMATOGRAPHY Meaning and Definition

1. Affinity chromatography is a powerful technique used in biochemistry and biotechnology to separate and purify specific biomolecules from complex mixtures. It relies on the specific interactions between a target molecule and a ligand immobilized on a chromatographic matrix.

   In affinity chromatography, the sample containing the mixture of molecules is passed through a column containing the chromatographic matrix, which is typically packed with small beads. These beads are modified with ligands that have a high affinity for the target molecule. The specific and reversible binding between the target molecule and the ligand allows for selective purification.

   The process begins with sample loading, where the complex mixture is applied to the column containing the matrix. Non-specific molecules are washed out with a buffer, while the target molecules, due to their affinity for the ligand, remain bound to the matrix. The specifically bound molecules are subsequently eluted by changing the conditions of the buffer, typically through the addition of a competitive inhibitor or by adjusting the pH or salt concentration.

   Affinity chromatography is widely used for the purification of proteins, antibodies, enzymes, and other biomolecules with high purity and yield. It facilitates the isolation of target molecules from a myriad of contaminants and background substances present in biological samples. This technique is especially valuable in the pharmaceutical industry where highly pure substances are required for drug development and production.

   In summary, affinity chromatography is a specialized separation technique that utilizes specific interactions between ligands and target molecules to selectively purify biomolecules from complex mixtures.

Common Misspellings for AFFINITY CHROMATOGRAPHY

• zffinity chromatography
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• afcinity chromatography
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• affunity chromatography
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• affknity chromatography
• affonity chromatography

Etymology of AFFINITY CHROMATOGRAPHY

The word "affinity" in the context of chromatography refers to the specific interaction that occurs between a target molecule (analyte) and a ligand (affinity ligand) that is immobilized on a solid support. The term "affinity" originates from the Latin word "affinitas", which means "relation by marriage" or "relationship". In chemistry, "affinity" describes the strength of the attraction or binding between two substances.

The term "chromatography" comes from the Greek words "chroma" (meaning "color") and "graphein" (meaning "to write"). The technique of chromatography was initially developed to separate and analyze pigments of different colors.

Therefore, the etymology of "affinity chromatography" can be understood as a technique in which the target analyte is separated and purified based on its specific binding affinity to a solid support containing an immobilized ligand.

Plural form of AFFINITY CHROMATOGRAPHY is AFFINITY CHROMATOGRAPHIES